7SYY

Hendra virus G protein head domain in complex with cross-neutralizing murine antibody hAH1.3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	137.253, 137.253, 149.967
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.440 - 2.740
R-factor	0.212
Rwork	0.211
R-free	0.23340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3d11
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20rc3_4406)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.440	10.000	2.850
High resolution limit [Å]	2.740	5.920	2.750
Rmerge	0.101	0.047	0.990
Number of reflections	38002	4068	3704
<I/σ(I)>	9.4
Completeness [%]	100.0	99.8	100
Redundancy	10.8	9.9	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	22% v/v Tacsimate pH 7.0, 0.1 M HEPES pH 7.5, 5% v/v Polyethylene glycol 200