7SWU
Crystal structure of the chromoprotein spisPINK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.825, 83.742, 88.469 |
| Unit cell angles | 90.00, 96.81, 90.00 |
Refinement procedure
| Resolution | 87.840 - 1.444 |
| R-factor | 0.1763 |
| Rwork | 0.175 |
| R-free | 0.19330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7swr |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.844 | 1.536 |
| High resolution limit [Å] | 1.444 | 1.444 |
| Rmerge | 0.130 | 1.606 |
| Rmeas | 0.140 | 1.732 |
| Rpim | 0.053 | 0.645 |
| Number of reflections | 157325 | 7867 |
| <I/σ(I)> | 8.8 | 1.3 |
| Completeness [%] | 94.1 | 56.9 |
| Redundancy | 7 | 7.1 |
| CC(1/2) | 0.997 | 0.523 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20 % (w/v) polyethylene glycol 3350, 0.2 M potassium nitrate |
