7SW9

MicroED structure of proteinase K from a 170 nm thick lamella measured at 300 kV

Experimental procedure

Collection date	2020-10-01
Spacegroup name	P 43 21 2
Unit cell lengths	68.600, 68.600, 104.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.440 - 2.100
R-factor	0.2756
Rwork	0.273
R-free	0.33200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cl7
RMSD bond length	0.002
RMSD bond angle	0.518
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.220
High resolution limit [Å]	2.100	2.100
Rmerge	0.668	1.776
Rmeas	0.746	1.977
Rpim	0.324
Number of reflections	68871	8977
<I/σ(I)>	3.38	0.96
Completeness [%]	89.9	85.5
Redundancy	5.1
CC(1/2)	0.917	0.294

Crystallization Conditions

crystal ID	method	pH	temperature	details
1