7SW3

MicroED structure of proteinase K from a 95 nm thick lamella measured at 200 kV

Experimental procedure

Collection date	2020-11-14
Spacegroup name	P 43 21 2
Unit cell lengths	67.950, 67.950, 102.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.980 - 2.350
R-factor	0.2022
Rwork	0.200
R-free	0.23990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cl7
RMSD bond length	0.004
RMSD bond angle	0.674
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.349
Rmeas	0.386	1.720
Rpim	0.159
Number of reflections	49966	4926
<I/σ(I)>	3.64	0.9
Completeness [%]	86.6	88.7
Redundancy	5.5
CC(1/2)	0.968	0.329

Crystallization Conditions

crystal ID	method	pH	temperature	details
1