7SW2

MicroED structure of proteinase K from a 130 nm thick lamella measured at 200 kV

Experimental procedure

Collection date	2020-11-14
Spacegroup name	P 43 21 2
Unit cell lengths	67.910, 67.910, 102.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.950 - 1.950
R-factor	0.1962
Rwork	0.194
R-free	0.23630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cl7
RMSD bond length	0.004
RMSD bond angle	0.793
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.261
Rmeas	0.295	1.675
Rpim	0.134
Number of reflections	84278	5983
<I/σ(I)>	4.4	0.8
Completeness [%]	96.8	98.4
Redundancy	4.8
CC(1/2)	0.979	0.382

Crystallization Conditions

crystal ID	method	pH	temperature	details
1