7SVJ
Bile Salt Hydrolase from Lactobacillus ingluviei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 90.636, 43.810, 155.060 |
| Unit cell angles | 90.00, 99.36, 90.00 |
Refinement procedure
| Resolution | 42.120 - 1.430 |
| Rwork | 0.177 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hez |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.821 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.120 | 1.480 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.040 | 0.979 |
| Rmeas | 0.057 | 1.384 |
| Rpim | 0.040 | 0.979 |
| Number of reflections | 108742 | 10677 |
| <I/σ(I)> | 8.5 | 0.8 |
| Completeness [%] | 97.5 | 95.9 |
| Redundancy | 1.9 | 1.9 |
| CC(1/2) | 0.999 | 0.413 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Calcium Acetate Hydrate, 0.1M Tris:HCl pH 7.0, 20% (w/v) PEG 3000. Crystals formed in a 1:2 protein (13.8 mg/mL) to mother liquor ratio. |
