7SVB
APE1 exonuclease substrate complex with 8oxoG opposite C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-24 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.205, 65.185, 91.330 |
| Unit cell angles | 90.00, 110.25, 90.00 |
Refinement procedure
| Resolution | 24.760 - 2.240 |
| R-factor | 0.2021 |
| Rwork | 0.200 |
| R-free | 0.24520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dfi |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX (1.19.2_4158) |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.290 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmeas | 0.133 | |
| Rpim | 0.658 | |
| Number of reflections | 65243 | 12091 |
| <I/σ(I)> | 10.1 | 1.72 |
| Completeness [%] | 99.6 | 98.4 |
| Redundancy | 4.1 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293.15 | 7 - 14% PEG20000, 100 mM sodium citrate, 15% glycerol, 5 mM calcium chloride |
