7SUU
Crystal Structure of Mouse Cadherin-23 EC25-26
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.237, 88.488, 206.783 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.055 - 2.248 |
| Rwork | 0.198 |
| R-free | 0.25330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vt8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.593 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.055 | 2.300 |
| High resolution limit [Å] | 2.248 | 2.248 |
| Number of reflections | 35493 | 1498 |
| <I/σ(I)> | 11.08 | 4.7 |
| Completeness [%] | 98.7 | 84.4 |
| Redundancy | 9.1 | 7.3 |
| CC(1/2) | 0.968 | 0.894 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 277 | 0.1 M HEPES ph 7.8 0.35 M CaCl2 28 % PEG 400 |
