7STS
Crystal Structure of Human Fab S24-1379 in the Complex with the N-teminal Domain of Nucleocapsid Protein from SARS CoV-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-14 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 99.084, 99.084, 221.890 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.540 - 2.160 |
| R-factor | 0.1996 |
| Rwork | 0.190 |
| R-free | 0.20860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | swisss model |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.527 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.460 | 2.200 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.088 | 2.210 |
| Number of reflections | 68533 | 3330 |
| <I/σ(I)> | 17.5 | 1.1 |
| Completeness [%] | 100.0 | 98.3 |
| Redundancy | 14.6 | 8.3 |
| CC(1/2) | 0.999 | 0.334 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 0.2 M Calcium chloride, 0.1 M Tris pH 8.0, 20 % (w/v) PEG 6000 |
