7ST4
Calcium-saturated jGCaMP8.410.80
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 120.597, 120.597, 97.876 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.390 - 2.000 |
| R-factor | 0.1762 |
| Rwork | 0.175 |
| R-free | 0.19730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ek4 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.732 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.240 | 47.240 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.057 | 0.029 | 0.514 |
| Rmeas | 0.060 | 0.030 | 0.535 |
| Rpim | 0.016 | 0.009 | 0.148 |
| Total number of observations | 707051 | 7553 | 46160 |
| Number of reflections | 49292 | 643 | 3583 |
| <I/σ(I)> | 31.3 | 71.7 | 5.6 |
| Completeness [%] | 100.0 | 98.7 | 100 |
| Redundancy | 14.3 | 11.7 | 12.9 |
| CC(1/2) | 1.000 | 1.000 | 0.949 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 298 | PEG 3350, potassium sodium tartrate tetrahydrate |
