7SR2

Crystal structure of the human SNX25 regulator of G-protein signalling (RGS) domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2021-02-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95364
Spacegroup name	P 1 21 1
Unit cell lengths	44.035, 75.864, 51.109
Unit cell angles	90.00, 109.27, 90.00

Refinement procedure

Resolution	41.570 - 2.420
R-factor	0.2144
Rwork	0.211
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7sr1
RMSD bond length	0.007
RMSD bond angle	0.904
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20rc3_4406)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.250	2.520
High resolution limit [Å]	2.420	2.420
Rpim	0.070	0.410
Number of reflections	11882	7264
<I/σ(I)>	7.2	1.4
Completeness [%]	97.9
Redundancy	6.8
CC(1/2)	0.992	0.631

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	18% PEG 8000, 0.1 M calcium acetate, 0.1 M sodium cacodylate, pH 6.5