7SR1

Crystal structure of the human SNX25 regulator of G-protein signalling (RGS) domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2017-02-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.006
Spacegroup name	P 1 21 1
Unit cell lengths	48.620, 57.140, 66.980
Unit cell angles	90.00, 109.48, 90.00

Refinement procedure

Resolution	45.840 - 2.400
R-factor	0.2174
Rwork	0.216
R-free	0.24210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bv1
RMSD bond length	0.003
RMSD bond angle	0.521
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20rc3_4406)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.840	2.486
High resolution limit [Å]	2.400	2.400
Rpim	0.061	0.467
Number of reflections	13200	1000
<I/σ(I)>	11.9	2.1
Completeness [%]	96.3	73.26
Redundancy	3.6	3.1
CC(1/2)	0.998	0.718

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	294	30% PEG 4000, 0.2 M Sodium acetate, 0.1 M Tris pH 8.5