7SQW
Structure of the KcsA-W67F mutant with the activation gate in the closed conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-09-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.2 |
Spacegroup name | I 4 |
Unit cell lengths | 153.962, 153.962, 75.804 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.690 - 3.210 |
R-factor | 0.2357 |
Rwork | 0.232 |
R-free | 0.27260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k4c |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.690 | 48.690 |
High resolution limit [Å] | 3.210 | 3.210 |
Number of reflections | 14014 | 14012 |
<I/σ(I)> | 14.7 | |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 3.1 | |
CC(1/2) | 0.750 | 0.500 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | PEG400 , 50 mM magnesium acetate, 50 mM sodium acetate, 300 mM KCl |