7SQW
Structure of the KcsA-W67F mutant with the activation gate in the closed conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.2 |
| Spacegroup name | I 4 |
| Unit cell lengths | 153.962, 153.962, 75.804 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.690 - 3.210 |
| R-factor | 0.2357 |
| Rwork | 0.232 |
| R-free | 0.27260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k4c |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.690 | 48.690 |
| High resolution limit [Å] | 3.210 | 3.210 |
| Number of reflections | 14014 | 14012 |
| <I/σ(I)> | 14.7 | |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.1 | |
| CC(1/2) | 0.750 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | PEG400 , 50 mM magnesium acetate, 50 mM sodium acetate, 300 mM KCl |
