7SQI

Crosslinked Crystal Structure of Type II Fatty Acid Synthase Ketosynthase, FabB, and C14-crypto Acyl Carrier Protein, AcpP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-07-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	58.990, 112.380, 141.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.100 - 1.700
Rwork	0.171
R-free	0.20520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6okc
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.100	39.100	1.730
High resolution limit [Å]	1.700	9.310	1.700
Rmerge	0.342	0.057	5.725
Rmeas	0.357	0.059	5.981
Rpim	0.102	0.016	1.706
Number of reflections	104223	744	5104
<I/σ(I)>	527
Completeness [%]	99.7	98.8	99.5
Redundancy	12	12.6	11.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	281	20% PEG 8000, 0.3 M sodium acetate, 0.1 M sodium cacodylate