7SOS
LaM domain of human LARP1 in complex with AAAA RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-09 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.96860 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.194, 46.799, 58.742 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.600 - 1.250 |
R-factor | 0.1756 |
Rwork | 0.174 |
R-free | 0.19480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7soo |
RMSD bond length | 0.016 |
RMSD bond angle | 1.667 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.270 |
High resolution limit [Å] | 1.250 | 1.250 |
Number of reflections | 28289 | 2236 |
<I/σ(I)> | 50.4 | |
Completeness [%] | 97.7 | |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.08M magnesium acetate, 1.344M potassium phosphate |