7SOQ

LaM domain of human LARP1 in complex with AAA RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	36.915, 46.474, 59.135
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.540 - 1.150
R-factor	0.1762
Rwork	0.176
R-free	0.18770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7soo
RMSD bond length	0.014
RMSD bond angle	1.431
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.170
High resolution limit [Å]	1.150	1.150
Number of reflections	35581	2885
<I/σ(I)>	32.2
Completeness [%]	96.8
Redundancy	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.056M sodium phosphate, 50mM sodium cacodylate, 30% PEG4000