7SOO

LaM domain of human LARP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.52154
Spacegroup name	P 21 21 21
Unit cell lengths	36.572, 46.776, 57.098
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.180 - 1.650
R-factor	0.2072
Rwork	0.205
R-free	0.22440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zh5
RMSD bond length	0.005
RMSD bond angle	0.794
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.710
High resolution limit [Å]	1.650	1.650
Number of reflections	12117	1138
<I/σ(I)>	55.9
Completeness [%]	98.7
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.2 M ammonium sulfate, 0.1 M Bis-Tris pH 6.5, 25% (w/v) PEG 3350