7SMD

p107 pocket domain complexed with EID1 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033
Spacegroup name	C 1 2 1
Unit cell lengths	98.741, 75.868, 71.973
Unit cell angles	90.00, 108.48, 90.00

Refinement procedure

Resolution	33.920 - 2.150
R-factor	0.229
Rwork	0.227
R-free	0.25940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yos
RMSD bond length	0.022
RMSD bond angle	2.236
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.920	2.220
High resolution limit [Å]	2.150	2.150
Rmerge	0.074	0.960
Number of reflections	27480	2385
<I/σ(I)>	11.5	1.8
Completeness [%]	99.6
Redundancy	3.3
CC(1/2)	0.990	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	100 mM MES, pH 6.5, 4% PEG400, 1.6 M ammonium sulfate