7SLZ

CRYSTAL STRUCTURE OF GID4 IN COMPLEX WITH BPF023596

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 41 21 2
Unit cell lengths	40.264, 40.264, 202.958
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.490 - 1.970
R-factor	0.208
Rwork	0.206
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wzz
RMSD bond length	0.010
RMSD bond angle	0.980
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.8.3)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.000
High resolution limit [Å]	1.970	5.350	1.970
Rmerge	0.050	0.033	0.975
Rmeas	0.052	0.034	1.012
Rpim	0.014	0.009	0.267
Total number of observations	179104
Number of reflections	12410	781	613
<I/σ(I)>	9.2
Completeness [%]	96.7	99.2	100
Redundancy	14.4	13.2	14.1
CC(1/2)		1.000	0.856

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	35% PEG 3350, 0.2M CaAC