7SLZ
CRYSTAL STRUCTURE OF GID4 IN COMPLEX WITH BPF023596
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 40.264, 40.264, 202.958 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.490 - 1.970 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6wzz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.980 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.8.3) |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.000 |
| High resolution limit [Å] | 1.970 | 5.350 | 1.970 |
| Rmerge | 0.050 | 0.033 | 0.975 |
| Rmeas | 0.052 | 0.034 | 1.012 |
| Rpim | 0.014 | 0.009 | 0.267 |
| Total number of observations | 179104 | ||
| Number of reflections | 12410 | 781 | 613 |
| <I/σ(I)> | 9.2 | ||
| Completeness [%] | 96.7 | 99.2 | 100 |
| Redundancy | 14.4 | 13.2 | 14.1 |
| CC(1/2) | 1.000 | 0.856 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 35% PEG 3350, 0.2M CaAC |
