7SKT
Crystal structure of Nipah virus matrix protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.445, 78.453, 135.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.610 - 2.048 |
| R-factor | 0.2161 |
| Rwork | 0.214 |
| R-free | 0.25610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bk6 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.950 |
| Data reduction software | XDS (Feb 5, 2021) |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 67.879 | 67.879 | 2.083 |
| High resolution limit [Å] | 2.048 | 5.557 | 2.048 |
| Rmerge | 0.073 | 0.025 | 1.047 |
| Rmeas | 0.081 | 0.028 | 1.154 |
| Rpim | 0.034 | 0.012 | 0.481 |
| Total number of observations | 221300 | 10730 | 11436 |
| Number of reflections | 40796 | 2187 | 2043 |
| <I/σ(I)> | 13.91 | 41.16 | 2.3 |
| Completeness [%] | 98.7 | 97.4 | 98.6 |
| Redundancy | 5.42 | 4.91 | 5.6 |
| CC(1/2) | 0.999 | 1.000 | 0.896 |
| Anomalous redundancy | 2.9 | 2.8 | 2.9 |
| CC(ano) | -0.225 | -0.338 | -0.049 |
| |DANO|/σ(DANO) | 0.7 | 0.6 | 0.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M TRIS pH 7.5, 20% PEG400 |
