7SKH

Crystal Structure of Mouse Cadherin-23 EC16-17

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-08
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	P 43 21 2
Unit cell lengths	90.861, 90.861, 170.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.267 - 2.265
Rwork	0.209
R-free	0.25170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wjm
RMSD bond length	0.012
RMSD bond angle	1.585
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.310
High resolution limit [Å]	2.265	2.265
Number of reflections	34006	1478
<I/σ(I)>	30.5	3
Completeness [%]	99.0	89.5
Redundancy	22.3	12.1
CC(1/2)	0.993	0.777

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	277	0.1 M MMT pH 9.0 25% PEG 1500