7SJV

Myocilin OLF mutant T353I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-04-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	49.365, 50.702, 50.644
Unit cell angles	90.00, 96.85, 90.00

Refinement procedure

Resolution	35.240 - 1.390
R-factor	0.1738
Rwork	0.173
R-free	0.18910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pke
RMSD bond length	0.005
RMSD bond angle	0.872
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.240	1.440
High resolution limit [Å]	1.390	1.390
Rmerge	0.123	0.557
Rmeas	0.134	0.617
Rpim	0.054	0.260
Number of reflections	49251	4798
<I/σ(I)>	16.62
Completeness [%]	98.3	96.03
Redundancy	6	4.8
CC(1/2)	0.997	0.892

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	289	8% PEG 8000, 0.1M magnesium chloride