7SH1
Class II UvrA protein - Ecm16
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-13 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 141.130, 141.130, 173.614 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 122.220 - 2.040 |
| R-factor | 0.19969 |
| Rwork | 0.198 |
| R-free | 0.23903 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vf8 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.990 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 173.614 | 2.076 |
| High resolution limit [Å] | 2.040 | 2.041 |
| Rmerge | 0.086 | 1.472 |
| Rmeas | 0.089 | |
| Rpim | 0.020 | 0.475 |
| Number of reflections | 127062 | 6279 |
| <I/σ(I)> | 23.5 | 2.3 |
| Completeness [%] | 100.0 | |
| Redundancy | 20.4 | |
| CC(1/2) | 0.999 | 0.796 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291.15 | 0.1 M MES pH 6.5, 0.2 M sodium thiocyanate, 12 % PEG (w/v) 20K |
