7SGX

Crystal Structure of Turtle Cadherin-23 EC1-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	P 43 21 2
Unit cell lengths	94.984, 94.984, 358.356
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.056 - 3.130
Rwork	0.253
R-free	0.31030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2whv
RMSD bond length	0.012
RMSD bond angle	1.313
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.200
High resolution limit [Å]	3.130	3.130
Rmeas	0.297	2.187
Number of reflections	30114	1456
<I/σ(I)>	12.559
Completeness [%]	99.8	100
Redundancy	12.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.7	277	0.1 M Tris pH 7.7 1.9 M CaCl2