7SGN
Crystal structure of periplasmic domain of Helicobacter pylori FliL (residues 81 to 183) (crystal form A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-06-15 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.501, 82.550, 97.840 |
| Unit cell angles | 67.72, 83.39, 72.82 |
Refinement procedure
| Resolution | 46.902 - 2.800 |
| R-factor | 0.1723 |
| Rwork | 0.168 |
| R-free | 0.25750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.965 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.160 | 2.910 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.103 | 0.390 |
| Number of reflections | 41760 | 4676 |
| <I/σ(I)> | 5.2 | |
| Completeness [%] | 98.0 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | Ammonium di-hydrogen phosphate and trisodium citrate |
