7SG1
XPA5 TCR in complex with HLA-DQ2-alpha1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-20 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.95370 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 137.602, 75.558, 216.804 |
Unit cell angles | 90.00, 103.76, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.420 | 3.200 |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 39655 | 3907 |
<I/σ(I)> | 6 | 1.4 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 2 | 2 |
CC(1/2) | 0.976 | 0.529 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | Mother liquor: 16-18% PEG3350, 0.12-0.14 M CaOAc, 0.1 M Tris/HCl at pH 8.0, Additives: 2 mM reduced and oxidised Glutathione |