7SFZ
Crystal structure of Mis18a-yippee domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 193.15 |
| Detector technology | PIXEL |
| Collection date | 2019-10-16 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.12713 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 110.725, 114.864, 116.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.985 - 3.002 |
| R-factor | 0.2058 |
| Rwork | 0.203 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hj0 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.294 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.105 | |
| Number of reflections | 30063 | 1479 |
| <I/σ(I)> | 16.5 | |
| Completeness [%] | 99.3 | |
| Redundancy | 4.4 | |
| CC(1/2) | 0.998 | 0.703 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295.15 | Ammonium sulfate, PEG 400, HEPES |
