7SFN
Crystal structure of OlmO, a spirocyclase involved in the biosynthesis of oligomycin
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-25 |
| Detector | DECTRIS PILATUS3 R 100K-A |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 65 |
| Unit cell lengths | 54.036, 54.036, 245.493 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.730 - 2.100 |
| R-factor | 0.1722 |
| Rwork | 0.171 |
| R-free | 0.20560 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | PHASER |
| Refinement software | PHENIX (18.1.3865) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.100 | 49.100 | 2.160 |
| High resolution limit [Å] | 2.100 | 8.900 | 2.100 |
| Rmerge | 0.074 | 0.036 | 0.711 |
| Rmeas | 0.078 | 0.038 | 0.760 |
| Rpim | 0.025 | 0.012 | 0.265 |
| Total number of observations | 221286 | 2991 | 15127 |
| Number of reflections | 23608 | 330 | 1862 |
| <I/σ(I)> | 17.6 | 45.1 | 3 |
| Completeness [%] | 99.6 | 99.9 | 95.1 |
| Redundancy | 9.4 | 9.1 | 8.1 |
| CC(1/2) | 0.999 | 0.999 | 0.863 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 3 M Malic acid, pH = 6.0 pH = 7.0 |
