7SFA
Branchiostoma floridae fluorescent protein LanFP10A2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-02-02 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 59.238, 96.217, 158.948 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.790 - 1.650 |
| R-factor | 0.1653 |
| Rwork | 0.165 |
| R-free | 0.19690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6m9x |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.880 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 3.550 | 1.650 |
| Rmerge | 0.080 | 0.029 | 0.781 |
| Rmeas | 0.088 | 0.031 | 0.856 |
| Rpim | 0.035 | 0.012 | 0.342 |
| Total number of observations | 624457 | ||
| Number of reflections | 107864 | 11489 | 10481 |
| <I/σ(I)> | 8 | ||
| Completeness [%] | 98.0 | 99.8 | 96.4 |
| Redundancy | 5.8 | 6.8 | 5.7 |
| CC(1/2) | 0.999 | 0.727 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 24% PEG 2000 MME, 0.08 M Tris |
