7SEW

M. tb EgtD in complex with HD6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2020-07-31
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	69.787, 69.787, 144.469
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.350 - 1.720
R-factor	0.1872
Rwork	0.184
R-free	0.22890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uy5
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.350	1.782
High resolution limit [Å]	1.720	1.720
Rmerge		0.350
Number of reflections	31266	1383
<I/σ(I)>	10
Completeness [%]	80.6
Redundancy	1
CC(1/2)	0.990	0.450

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	0.2 M sodium sulfate and 20 % w/v polyethylene glycol 1,000