7SEV

Crystal structure of E coli contaminant protein YadF co-purified with a plant protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.891
Spacegroup name	P 42 21 2
Unit cell lengths	67.500, 67.500, 85.212
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.030 - 2.300
R-factor	0.2059
Rwork	0.204
R-free	0.24270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold AF-P61517-F1
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.030	2.360
High resolution limit [Å]	2.300	2.300
Number of reflections	16710	665
<I/σ(I)>	9.9
Completeness [%]	100.0
Redundancy	24
CC(1/2)	0.995	0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M sodium cacodylate, pH 6.8 and 1.8 M (NH4)2SO4