7SES

PRMT5/MEP50 with compound 29 bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-20
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.979
Spacegroup name	I 2 2 2
Unit cell lengths	104.783, 138.053, 179.034
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.250 - 2.500
R-factor	0.2116
Rwork	0.210
R-free	0.24490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7s0u
RMSD bond length	0.002
RMSD bond angle	0.526
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Rpim	0.060	0.290
Number of reflections	42618	2048
<I/σ(I)>	10.2
Completeness [%]	93.9
Redundancy	2.8
CC(1/2)		0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	100 mM Sodium Citrate pH 5, 10% PEG 4K, 50 mM Sodium Acetate (pH 7)