7SEJ

Structure-based design of prefusion-stabilized human metapneumovirus fusion proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-11-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	58.379, 105.809, 90.990
Unit cell angles	90.00, 105.26, 90.00

Refinement procedure

Resolution	45.310 - 2.510
R-factor	0.2113
Rwork	0.209
R-free	0.25270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wb0
RMSD bond length	0.005
RMSD bond angle	0.700
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.400	2.600
High resolution limit [Å]	2.500	2.510
Rmerge	0.078	0.038
Rpim	0.073	0.037
Number of reflections	35650	801
<I/σ(I)>	8.3	15.5
Completeness [%]	97.6
Redundancy	3.2
CC(1/2)	0.994	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	500 nl of hMPV F (10 mg/ml) with 500 nl of reservoir solution containing 0.1 M MES pH 6.0 and 12%(v/v) PEK 20k.