7SDP
Replication Initiator Protein REPE54 and cognate DNA sequence with terminal three prime phosphates.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2021-04-16 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 109.142, 83.089, 74.593 |
Unit cell angles | 90.00, 122.69, 90.00 |
Refinement procedure
Resolution | 37.680 - 3.010 |
R-factor | 0.1848 |
Rwork | 0.182 |
R-free | 0.23510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7rva |
RMSD bond length | 0.012 |
RMSD bond angle | 1.355 |
Data reduction software | XDS (Jan 31, 2020) |
Data scaling software | XSCALE (Jan 31, 2020) |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.680 | 3.190 |
High resolution limit [Å] | 3.010 | 3.010 |
Rmerge | 0.140 | 0.895 |
Rmeas | 0.165 | |
Rpim | 0.086 | 0.556 |
Number of reflections | 11131 | 1781 |
<I/σ(I)> | 7.2 | 1.3 |
Completeness [%] | 99.1 | 97.7 |
Redundancy | 3.7 | 3.5 |
CC(1/2) | 0.993 | 0.740 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 100 mM MgCl2, 7% PEG 400, 220 mM Tris HCl pH 8.0 |