7SCW

KRAS full length wild-type in complex with RGL1 Ras association domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92
Spacegroup name	P 63 2 2
Unit cell lengths	91.064, 91.064, 132.896
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.450 - 1.980
R-factor	0.1578
Rwork	0.156
R-free	0.19210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lfd
RMSD bond length	0.016
RMSD bond angle	1.378
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.450	2.050
High resolution limit [Å]	1.980	1.980
Rmerge	0.177	1.096
Rmeas	0.181	1.134
Rpim	0.041	0.284
Number of reflections	23259	2184
<I/σ(I)>	20.4	4.4
Completeness [%]	99.6	96.4
Redundancy	19.2
CC(1/2)	0.999	0.867

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	0.05M Magnesium acetate tetrahydrate, 0.1M MES (pH 6.5), 26% v/v PEG 400