7SCP
The crystal structure of ScoE in complex with intermediate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97917 |
Spacegroup name | P 42 |
Unit cell lengths | 96.820, 96.820, 69.840 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.890 - 1.990 |
R-factor | 0.2293 |
Rwork | 0.227 |
R-free | 0.25340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6l6x |
RMSD bond length | 0.002 |
RMSD bond angle | 0.442 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (v1.18.2-3874) |
Refinement software | PHENIX (v1.18.2-3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.890 | 2.040 |
High resolution limit [Å] | 1.990 | 1.990 |
Number of reflections | 44184 | 3076 |
<I/σ(I)> | 19.5 | |
Completeness [%] | 99.4 | |
Redundancy | 13.5 | |
CC(1/2) | 0.999 | 0.725 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD 0.03 M of each divalent cation 0.1 M MOPS/HEPES-Na pH 7.5 |