7SA9

Human MUC16 SEA5 Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.87
Spacegroup name	C 1 2 1
Unit cell lengths	78.540, 90.145, 42.821
Unit cell angles	90.00, 102.88, 90.00

Refinement procedure

Resolution	58.360 - 1.690
R-factor	0.2009
Rwork	0.200
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ivz
RMSD bond length	0.004
RMSD bond angle	0.670
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.360	1.751
High resolution limit [Å]	1.690	1.690
Rmerge	0.060	0.620
Rmeas	0.072	0.397
Rpim	0.039
Number of reflections	32113	10606
<I/σ(I)>	9.74	1.4
Completeness [%]	98.6	97.3
Redundancy	3.3	3.3
CC(1/2)	0.996	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	ammonium acetate, PEG 10000