7SA8
Crystal Structure of the periplasmic lyase AlgL K66A Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2015-05-13 |
| Detector | BRUKER PHOTON 100 |
| Wavelength(s) | 1.5406 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 58.736, 67.496, 76.216 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.305 - 2.500 |
| Rwork | 0.246 |
| R-free | 0.26890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ozv |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.503 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.305 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.098 | 0.435 |
| Number of reflections | 10903 | 1196 |
| <I/σ(I)> | 16 | |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.275 M K2SO4, 19% (w/v) PEG 3350, and 0.1 M HEPES pH 6.9. Crystals were cryoprotected by soaking for 10 min with 2 mM mannuronate tetrasaccharide and 20% (v/v) PEG 4000. |
