7SA1
LRR-F-Box plant ubiquitin ligase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-04-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 231.929, 79.407, 150.595 |
| Unit cell angles | 90.00, 127.98, 90.00 |
Refinement procedure
| Resolution | 48.900 - 3.210 |
| R-factor | 0.211 |
| Rwork | 0.208 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6brp |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.454 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.900 | 3.320 |
| High resolution limit [Å] | 3.210 | 3.210 |
| Number of reflections | 34685 | 3119 |
| <I/σ(I)> | 22.7 | |
| Completeness [%] | 97.6 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.1M Ammonium acetate, 0.1M Sodium formate, 0.1M Sodium citrate tribasic dihydrate, 0.1M Potassium sodium tartrate tetrahydrate, 0.1M Sodium oxamate, 0.1M Tris, 0.1M Bicine and 30% PEG 500 MME and PEG 20000 at pH 8.5. |
