7S9J
Crystal Structure of DNA Polymerase Beta with Fapy-dG base-paired with a dC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-08-20 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.383, 79.494, 54.885 |
Unit cell angles | 90.00, 105.72, 90.00 |
Refinement procedure
Resolution | 24.860 - 1.910 |
R-factor | 0.1911 |
Rwork | 0.188 |
R-free | 0.23790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3isb |
RMSD bond length | 0.010 |
RMSD bond angle | 1.024 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 5.170 |
High resolution limit [Å] | 1.910 | 1.910 |
Rmeas | 0.122 | |
Number of reflections | 53238 | 1730 |
<I/σ(I)> | 11 | |
Completeness [%] | 99.6 | |
Redundancy | 4.4 | |
CC(1/2) | 0.997 | 0.608 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 14-19% PEG 3350, 50 mM Imidazole pH 8.0, 350 mM Sodium Acetate |