7S97

Structure of the Photoacclimated Light Harvesting Complex PC577 from Hemiselmis pacifica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-02
Detector	DECTRIS PILATUS3 R 300K
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	41.131, 95.736, 124.887
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.680 - 2.350
R-factor	0.1895
Rwork	0.186
R-free	0.25690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7s96
RMSD bond length	0.007
RMSD bond angle	0.911
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.390
High resolution limit [Å]	2.350	6.360	2.350
Rmerge	0.159	0.065	0.776
Rmeas	0.180	0.069	0.926
Rpim	0.081	0.024	0.492
Total number of observations	90326
Number of reflections	20796	1194	991
<I/σ(I)>	4.8
Completeness [%]	97.5	99.9	95
Redundancy	4.3	7.8	3
CC(1/2)		0.998	0.696

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25% 3350 PEG, 50 mM HEPES, 100 mM MgCl2