7S8Q
Crystal Structure of HLA A*1101 in complex with KTNGNAFIGK, an 10-mer epitope from Influenza B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-17 |
| Detector | DECTRIS PILATUS 12M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 43 |
| Unit cell lengths | 119.481, 119.481, 63.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.870 - 2.080 |
| R-factor | 0.175 |
| Rwork | 0.173 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mj5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.500 | 2.140 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Number of reflections | 53875 | 3912 |
| <I/σ(I)> | 11.1 | 2 |
| Completeness [%] | 99.5 | 93.5 |
| Redundancy | 13.8 | 12.3 |
| CC(1/2) | 0.997 | 0.589 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 20% PEG 3350, 0.1M BisTris pH 6.5 |
