7S7S

Crystal structure of hydrophobin SC16, P21212

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER D8 QUEST
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-20
Detector	Bruker PHOTON II
Wavelength(s)	1.5406
Spacegroup name	P 21 21 2
Unit cell lengths	48.487, 37.665, 43.761
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.210 - 2.200
Rwork	0.171
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nbh
RMSD bond length	0.011
RMSD bond angle	1.166
Data reduction software	PROTEUM2
Data scaling software	pointless
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.881	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.196	0.783
Rmeas	0.198	0.790
Rpim	0.026	0.101
Number of reflections	4374	422
<I/σ(I)>	21.4	6.3
Completeness [%]	100.0	100
Redundancy	58.3
CC(1/2)	0.999	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.5	293	0.1 M Citric Acid pH 3.5 29% PEG 3350