7S7R

Plasmodium falciparum protein Pf12 bound to nanobody G7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 32 2 1
Unit cell lengths	50.931, 50.931, 322.764
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.703 - 1.770
R-factor	0.2223
Rwork	0.221
R-free	0.25520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ymo 7kjh
RMSD bond length	0.012
RMSD bond angle	1.214
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.109	46.109	1.880
High resolution limit [Å]	1.770	5.270	1.770
Rmerge	0.065	0.029	1.117
Rmeas	0.068	0.030	1.154
Total number of observations	740569
Number of reflections	48590	2116	7787
<I/σ(I)>	22.05	75.36	1.84
Completeness [%]	98.7	99.8	99.9
Redundancy	15.241	13.078	15.909
CC(1/2)	1.000	1.000	0.935

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		281	15% (w/v) PEG 4000, 200 mM potassium thiocyanate, 0.05 M sodium cacodylate pH 6