7S7H

Complex structure of Methane monooxygenase hydroxylase and regulatory subunit DBL2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	102.516, 106.357, 296.685
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.170 - 2.400
R-factor	0.2213
Rwork	0.219
R-free	0.26060
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	7m8q
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	148.340	148.340	2.440
High resolution limit [Å]	2.400	13.150	2.400
Rmerge	0.135	0.060	1.028
Rmeas	0.154	0.069	1.180
Rpim	0.072	0.034	0.561
Total number of observations	427902	2977	19178
Number of reflections	119799	832	5529
<I/σ(I)>	5.6	15.2	0.7
Completeness [%]	94.4	93.7	88.6
Redundancy	3.6	3.6	3.5
CC(1/2)	0.991	0.994	0.653

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.8	295	20% PEG3350, 0.2 M sodium phosphate dibasic, pH 8.8