7S7G
Crystal Structure Analysis of Human VLCAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 75.260, 108.270, 149.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.120 - 1.340 |
| R-factor | 0.1471 |
| Rwork | 0.146 |
| R-free | 0.16850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b96 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 57.120 | 57.170 | 1.360 |
| High resolution limit [Å] | 1.340 | 3.640 | 1.340 |
| Rmeas | 0.055 | 0.040 | 1.692 |
| Rpim | 0.021 | 0.015 | 0.656 |
| Total number of observations | 891338 | 45652 | 44299 |
| Number of reflections | 135832 | 7080 | 6769 |
| <I/σ(I)> | 15.5 | 45.7 | 1.2 |
| Completeness [%] | 99.5 | 98.9 | 100 |
| Redundancy | 6.6 | 6.4 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 300 | 100 mM sodium citrate, pH 5.5, 11% PEG2000 MME |
