7S6R
Complex structure of Methane monooxygenase hydroxylase and regulatory subunit with H5A mutation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 102.655, 105.907, 299.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 73.710 - 1.890 |
R-factor | 0.1624 |
Rwork | 0.161 |
R-free | 0.19470 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 7m8q |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 149.560 | 149.560 | 1.920 |
High resolution limit [Å] | 1.890 | 10.330 | 1.890 |
Rmerge | 0.107 | 0.061 | 1.010 |
Rmeas | 0.116 | 0.067 | 1.095 |
Rpim | 0.045 | 0.027 | 0.419 |
Total number of observations | 10965 | 77436 | |
Number of reflections | 261001 | 1810 | 11780 |
<I/σ(I)> | 10.5 | 28.5 | 1.5 |
Completeness [%] | 99.5 | 99.6 | 91.6 |
Redundancy | 6.7 | 6.1 | 6.6 |
CC(1/2) | 0.997 | 0.997 | 0.732 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 295 | 20% PEG3350, 0.2 M sodium phosphate dibasic, pH 8.8 |