7S6Q

Complex structure of Methane monooxygenase hydroxylase and regulatory subunit DBL2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-13
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	102.176, 105.358, 297.809
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	148.900 - 1.960
R-factor	0.1879
Rwork	0.186
R-free	0.22670
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	7m8r
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	148.900	148.900	1.990
High resolution limit [Å]	1.960	10.740	1.960
Rmerge	0.118	0.045	1.800
Rmeas	0.142	0.054	2.164
Rpim	0.076	0.029	1.179
Total number of observations	705096	4706	35457
Number of reflections	225895	1514	11236
<I/σ(I)>	6.7	24.4	0.6
Completeness [%]	98.3	96	99.7
Redundancy	3.1	3.1	3.2
CC(1/2)	0.993	0.997	0.292

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.8	295	20% PEG3350, 0.2 M sodium phosphate dibasic, pH 8.8