7S5U
Extended bipolar assembly domain of kinesin-5 minifilament
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 253.180, 84.890, 96.770 |
| Unit cell angles | 90.00, 91.44, 90.00 |
Refinement procedure
| Resolution | 96.735 - 4.410 |
| R-factor | 0.2767 |
| Rwork | 0.274 |
| R-free | 0.30970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pxt |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 96.740 | 96.735 | 4.640 |
| High resolution limit [Å] | 4.400 | 13.910 | 4.400 |
| Rmerge | 0.043 | 0.473 | |
| Rmeas | 0.133 | 0.051 | 0.605 |
| Rpim | 0.064 | 0.024 | 0.292 |
| Total number of observations | 1711 | 7964 | |
| Number of reflections | 13060 | 436 | 1861 |
| <I/σ(I)> | 4.6 | 20.5 | 1.8 |
| Completeness [%] | 98.7 | 96.9 | 98.8 |
| Redundancy | 4.3 | 3.9 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 292 | 0.01 M FeCl3, 0.1M sodium citrate pH 5.6, 12% Jeffamine M-600 |
