7S5L
Cembrene A synthase from Eleutherobia rubra
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 85.689, 101.649, 46.342 |
| Unit cell angles | 90.00, 90.60, 90.00 |
Refinement procedure
| Resolution | 37.970 - 1.580 |
| R-factor | 0.1631 |
| Rwork | 0.162 |
| R-free | 0.19200 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.709 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18_3845) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.970 | 1.670 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Number of reflections | 52295 | 8256 |
| <I/σ(I)> | 14.7 | |
| Completeness [%] | 96.1 | |
| Redundancy | 3.9 | |
| CC(1/2) | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 300 | 0.1 M Tris pH 8.5, 28% (w/V) PEG 3350, 0.28 M MgCl2 |
